Regulatory

Part:BBa_K511003:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)

UAS-Gal4 Promoter MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable.


Source

This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References